Axially overlapped multi-focus light sheet with enlarged field of view

Light sheet fluorescence microscopy provides optical sectioning and is widely used in volumetric imaging of large specimens. However, the axial resolution lateral Field View (FoV) system, defined by light sheet, typically limit each other due to spatial band product excitation objective. Here, we develop a simple multi-focus scheme extend FoV, where Gaussian can be focused at three or more consecutive positions. Axially overlapped multiple sheets significantly enlarge FoV with improved uniformity negligible loss resolution. By measuring point spread function fluorescent beads, demonstrated that obtained has 450 μm maximum FWHM 7.5 μm. Compared conventional single-focus one, displays ability over full when cells zebrafish.